ncRNA basic information
ncRNA ID:
MI0003188
ncRNA Database:
miRBase
ncRNA Name:
miR-503
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
PI3K/Akt pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
MTS assay was employed to determine the effect of miR-503 on A549/DDP sensitivity to cisplatin. Apoptosis rate and intracellular concentration of rhodamine-123 (Rh-123) were determined by flow cytometry, the expression of multi-drugs resistant proteins MDR1 and MRP1, ERCC1, RhoE, Survivin and Bcl-2 were determined by Western blot and real time PCR. The phosphorylation of Akt was analyzed by Western blot, the transcriptional activities of NF-kappaB and AP-1 were detected by dual-luciferase reporter gene systems.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549,A549/DDP)
Other information
Title:
The reversing and molecular mechanisms of miR-503 on the drug-resistance to cisplatin in A549/DDP cells
Journal:
Zhongguo Fei Ai Za Zhi
Published:
2014
PubMed ID:
24398307