ncRNA basic information
ncRNA ID:
MI0003175
ncRNA Database:
miRBase
ncRNA Name:
miR-520h
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
HDAC1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.The cell viability was measured by MTS .
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (MKN45, MKN28)
Other information
Title:
Downregulation of histone deacetylase 1 by microRNA-520h contributes to the chemotherapeutic effect of doxorubicin.
Journal:
FEBS Lett
Published:
2014
PubMed ID:
24316511