ncRNA basic information
ncRNA ID:
MI0000089
ncRNA Database:
miRBase
ncRNA Name:
miR-31
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real-time quantitative RT-PCR
ncRNA Target Gene:
ABCB9
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. The apoptosis was analyzed on a FACS Calibur flow cytometer using an Annexin V-FITC/PI .
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line (SPC-A-1, LTEP-a-2, NCI-H460, NCI-H1299 )
Other information
Title:
MicroRNA-31 inhibits cisplatin-induced apoptosis in non-small cell lung cancer cells by regulating the drug transporter ABCB9.
Journal:
Cancer Lett
Published:
2014
PubMed ID:
24099915