ncRNA basic information
ncRNA ID:
MI0000462
ncRNA Database:
miRBase
ncRNA Name:
miR-152
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
DNMT1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.Flow cytometry analysis was used to determine if cells were viable, apoptotic, or necrotic. Nude mice model was used to idetify the role of miR-152 and miR-185 .
Drug Response:
sensitive
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line ( SKOV3, A2780,A2780/DDP,SKOV3/DDP)
Other information
Title:
MiR-152 and miR-185 co-contribute to ovarian cancer cells cisplatin sensitivity by targeting DNMT1 directly: a novel epigenetic therapy independent of decitabine.
Journal:
Oncogene
Published:
2014
PubMed ID:
23318422