ncRNA basic information
ncRNA ID:
MI0000342
ncRNA Database:
miRBase
ncRNA Name:
miR-200b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
SUZ12 and ROCK2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00544 (APRD00516, EXPT03204)
Drug Name:
Fluorouracil
Drug Method:
In this study, we identified dysregulated microRNAs (miRNAs, miR) in cholangiocarcinoma tissue by microarray analysis, and subsequent real-time PCR and northern blot analyses validated the expression of candidate miR. We performed functional analyses and investigated the relationship between miR-200b/c expression and the properties of cholangiocarcinoma cells. A dual luciferase assay was applied to examine the effect of miRNAs on the 3'-UTR of target genes, and we demonstrated the function of the target gene by siRNA transfection identifying the downstream pathway via western blotting.
Drug Response:
sensitive
Cancer basic information
Cancer:
cholangiocarcinoma
Tissue/Cell:
tissue and cell line (TFK-1, QBC939)
Other information
Title:
Direct targeting of SUZ12/ROCK2 by miR-200b/c inhibits cholangiocarcinoma tumourigenesis and metastasis.
Journal:
Br J Cancer
Published:
2013
PubMed ID:
24169343