ncRNA basic information
ncRNA ID:
MI0000448
ncRNA Database:
miRBase
ncRNA Name:
miR-130a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
XIAP
ncRNA Pathway:
miR-130a/XIAP signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs.Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line (A2780,A2780/DDP )
Other information
Title:
Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly.
Journal:
Acta Biochim Biophys Sin (Shanghai)
Published:
2013
PubMed ID:
24145606