ncRNA basic information
ncRNA ID:
MI0000066
ncRNA Database:
miRBase
ncRNA Name:
let-7e
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
EZH2 and CCND1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time PCR and those of protein were by Western blot analysis. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Atomic absorption spectroscopy for detection of cisplatin in tumor tissue . The viability of cells treated with cisplatin after transfection with let-7e agomirs was assessed by Trypan blue exclusion method.
Drug Response:
resistant
Cancer basic information
Cancer:
epithelial ovarian cancer
Tissue/Cell:
cell line (A2780, ES2, SKOV3,A2780/CP)
Other information
Title:
Deregulation of let-7e in epithelial ovarian cancer promotes the development of resistance to cisplatin.
Journal:
Oncogenesis
Published:
2013
PubMed ID:
24100610