ncRNA basic information
ncRNA ID:
MI0000076
ncRNA Database:
miRBase
ncRNA Name:
miR-20a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
EGR2
ncRNA Pathway:
EGR2 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Quantitative Real Time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.Cell migration was detected by transwell migration and invasion assay.
Drug Response:
resistant
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line ( SGC7901, MKN45, NUGC-3,GES-1)
Other information
Title:
Involvement of miR-20a in promoting gastric cancer progression by targeting early growth response 2 (EGR2).
Journal:
Int J Mol Sci
Published:
2013
PubMed ID:
23924943