ncRNA basic information
ncRNA ID:
MI0003188
ncRNA Database:
miRBase
ncRNA Name:
miR-503
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Bcl-2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow cytometry was used to detect the apoptosis .
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line (A549,A549/CDDP)
Other information
Title:
miR-503 regulates the resistance of non-small cell lung cancer cells to cisplatin by targeting Bcl-2.
Journal:
Int J Mol Med
Published:
2013
PubMed ID:
23856992