ncRNA basic information
ncRNA ID:
MI0000489
ncRNA Database:
miRBase
ncRNA Name:
miR-195
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Raf-1
ncRNA Pathway:
Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
The expression levels of miRNA were determined by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and those of protein were by Western blot analysis. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. Prediction of miRNA targeting genes by computer-based miRNA target detection programs .
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
tissue and cell line (MCF-7,HBL-100,MCF-7/ADR,)
Other information
Title:
Upregulation of miR-195 increases the sensitivity of breast cancer cells to Adriamycin treatment through inhibition of Raf-1.
Journal:
Oncol Rep
Published:
2013
PubMed ID:
23760062