ncRNA basic information
ncRNA ID:
MI0006353
ncRNA Database:
miRBase
ncRNA Name:
miR-1291
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ABCC1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Reverse Transcription Quantitative Real-Time PCR (qPCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
pancreatic carcinoma
Tissue/Cell:
cell line (PANC-1,H69,H69AR,HEK-293 )
Other information
Title:
Small nucleolar RNA-derived microRNA hsa-miR-1291 modulates cellular drug disposition through direct targeting of ABC transporter ABCC1.
Journal:
Drug Metab Dispos
Published:
2013
PubMed ID:
23686318