ncRNA basic information
ncRNA ID:
MI0000268
ncRNA Database:
miRBase
ncRNA Name:
miR-34
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Bcl-2,Notch,HMGA2
ncRNA Pathway:
p53 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Caspase-3 and -7 activation status was measured using the Caspase-Glo 3/7 Assay.
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (Kato III, AGS, N87, MKN45,WI-38)
Other information
Title:
Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres.
Journal:
BMC Cancer
Published:
2008
PubMed ID:
18803879