ncRNA basic information ncRNA ID: hsa_circ_0025202 ncRNA Database: circBase ncRNA Name: hsa_circ_0025202 ncRNA Type: circRNA ncRNA Expression: down-regulated ncRNA Method: qRT-PCR ncRNA Target Gene: FOXO3a ncRNA Pathway: NA Evidence (ncRNA-drug): validated

drug basic information Drug ID: DB00675 (APRD00123) Drug Name: Tamoxifen Drug Method: To investigate roles of circular RNAs (circRNAs) in tamoxifen resistance, a tamoxifen-resistant MCF-7 cell line was established and screened for its circRNA expression profile by RNA sequencing.hsa_circ_0025202, a circRNA that was significantly downregulated, was selected for further investigation.Using a large cohort of clinical specimens, we found that hsa_circ_0025202 exhibited low expression in cancer tissues and was negatively correlated with lymphatic metastasis and histological grade.Gain- and loss-of-function assays indicated that hsa_circ_0025202 could inhibit cell proliferation, colony formation, and migration and increase cell apoptosis and sensitivity to tamoxifen.Bioinformatics and luciferase reporter assays verified that hsa_circ_0025202 could act as a miRNA sponge for miR-182-5p and further regulate the expression and activity of FOXO3a.Functional studies revealed that tumor inhibition and tamoxifen sensitization effects of hsa_circ_0025202 were achieved via the miR-182-5p/FOXO3a axis.Foreover, in vivo experiments confirmed that hsa_circ_0025202 could suppress tumor growth and enhance tamoxifen efficacy. Drug Response: resistant

Cancer basic information Cancer: breast cancer Tissue/Cell: cell line (MCF7 and T47D and HEK293T)

Other information Title: circRNA_0025202 Regulates Tamoxifen Sensitivity and Tumor Progression via Regulating the miR-182-5p/FOXO3a Axis in Breast Cancer. Journal: Mol Ther Published: 2019 PubMed ID: 31153828