ncRNA basic information
ncRNA ID:
MI0000298
ncRNA Database:
miRBase
ncRNA Name:
miR-221
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR, RT-PCR
ncRNA Target Gene:
PTEN
ncRNA Pathway:
PI3K/Akt pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR and RT-PCR . Those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.Cell cycle analysis and apoptosis assay by flow cytometry.Caspase-3 activation status was measured using the Caspase-Glo 3 Assay .
Drug Response:
resistant
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (SOSP-9607, SOSP-9901,MG63, Saos-2, U2OS, hFOB1.19)
Other information
Title:
MicroRNA-221 induces cell survival and cisplatin resistance through PI3K/Akt pathway in human osteosarcoma.
Journal:
PLoS One
Published:
2013
PubMed ID:
23372675