ncRNA basic information
ncRNA ID:
MI0000289/MI0000269
ncRNA Database:
miRBase
ncRNA Name:
miR-181a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
PTEN,KRAS,RB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00541 (APRD00495)
Drug Name:
Vincristine
Drug Method:
The HCC Huh-7 cell line was treated with adramycin (ADM), cisplatin (DDP), carboplatin (CBP), mitomycin C (MMC) or vincristine (VCR) at increasing concentrations to develop drug-resistant sublines. The cell viability MTT assay was used to detect drug resistance. Five different drug-resistant HCC sublines, Huh-7/ADM, Huh-7/CBP, Huh-7/DDP, Huh-7/MMC and Huh-7/VCR, were established. Cells that were resistant to one drug were also found to be resistant to the other drugs. miRNA microarrays were analyzed to identify differential miRNA expression profiles in these cell lines, and real-time PCR was used to validate miRNA microarray data.
Drug Response:
resistant
Cancer basic information
Cancer:
hepatocellular carcinoma
Tissue/Cell:
cell line (Huh-7,Huh-7/ADM,Huh-7/CBP,Huh-7/DDP,Huh-7/MMC,Huh-7/VCR)
Other information
Title:
Differential miRNA expression profiles in hepatocellular carcinoma cells and drug-resistant sublines.
Journal:
Oncol Rep
Published:
2013
PubMed ID:
23229111