ncRNA basic information
ncRNA ID:
MI0000268
ncRNA Database:
miRBase
ncRNA Name:
miR-34a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Notch1
ncRNA Pathway:
Notch signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
The role of miR-34a in breast cancer cells was detected using MTT assay, flow cytometry assay, real-time PCR and Western blot, etc. The association of miR-34a and Notch1 was analyzed by dual-luciferase reporter assay and Notch1-siRNA technology. Real-time PCR assay was performed to test the expression of miR-34a and Notch1 in 38 selective breast cancer tissue samples.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7,MCF-7/ADR,MCF-7/DOC)
Other information
Title:
MicroRNA-34a modulates chemosensitivity of breast cancer cells to adriamycin by targeting Notch1.
Journal:
Arch Med Res
Published:
2012
PubMed ID:
23085450