ncRNA basic information
ncRNA ID:
MI0000077
ncRNA Database:
miRBase
ncRNA Name:
miR-21
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
PTEN
ncRNA Pathway:
PI3K/Akt/mTOR signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
chronic myeloid leukemia
Tissue/Cell:
cell line (K562, K562/A02)
Other information
Title:
Triptolide modulates the sensitivity of K562/A02 cells to adriamycin by regulating miR-21 expression.
Journal:
Pharm Biol
Published:
2012
PubMed ID:
22957792