ncRNA basic information
ncRNA ID:
MI0001446
ncRNA Database:
miRBase
ncRNA Name:
miR-424
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
WEE1 and CHK1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In this study, we conducted parallel high-throughput screens for microRNAs (miRNA) that could restore sensitivity to cisplatin-resistant cells, and we screened for kinases targeted by miRNAs that mediated cisplatin resistance. The expression levels of miRNA were determined by quantitative real-time (RT)-PCR and those of protein were by Western blot analysis. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. miRNAs predicted to target kinases identified by the high throughput screen were identified by using miRanda and TargetScanHuman .
Drug Response:
sensitive
Cancer basic information
Cancer:
epidermoid carcinoma
Tissue/Cell:
cell line(KB-3-1,KB-CP.5,KB-CP20)
Other information
Title:
Cisplatin sensitivity mediated by WEE1 and CHK1 is mediated by miR-155 and the miR-15 family.
Journal:
Cancer Res
Published:
2012
PubMed ID:
22942255