ncRNA basic information
ncRNA ID:
MIMAT0000231
ncRNA Database:
miRBase
ncRNA Name:
miR-199a-5p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
ATG7
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time polymerase chain reaction (PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs.The CCK-8 assay was used to monitor the growth of the cells.
Drug Response:
resistant
Cancer basic information
Cancer:
hepatocellular carcinoma
Tissue/Cell:
cell line ( (Huh7, HepG2,HEK 293)
Other information
Title:
Cisplatin-induced downregulation of miR-199a-5p increases drug resistance by activating autophagy in HCC cell.
Journal:
Biochem Biophys Res Commun
Published:
2012
PubMed ID:
22713463