ncRNA basic information
ncRNA ID:
MI0000299
ncRNA Database:
miRBase
ncRNA Name:
miR-222
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ADAM-17
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00541 (APRD00495)
Drug Name:
Vincristine
Drug Method:
The expression levels of miRNA were determined by Quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Flow cytometric analysis of apoptosis.MiRNA microarray analysis was used to identify the expression in different cell lines.
Drug Response:
resistant
Cancer basic information
Cancer:
colorectal carcinoma
Tissue/Cell:
cell line (HCT-8, HCT-116,HCT-8/VCR, HCT-116/L-OHP)
Other information
Title:
MiR-222 modulates multidrug resistance in human colorectal carcinoma by down-regulating ADAM-17.
Journal:
Exp Cell Res
Published:
2012
PubMed ID:
22677042