ncRNA basic information
ncRNA ID:
MI0000448
ncRNA Database:
miRBase
ncRNA Name:
miR-130a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real-time qRT-PCR
ncRNA Target Gene:
PTEN
ncRNA Pathway:
PI3K/Akt/PTEN/mTOR
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The aim of this study was to investigate microRNA expression profiles in cisplatin-resistant ovarian cancer cells and the role of miR-130a in regulating drug resistance. Analysis of differentially expressed miRNAs between SKOV3 and SKOV3/CIS cells was assessed by miRNA microarrays. Target prediction of miRNAs was determined with the help of PicTar or TargetScan. Among these miRNAs, the expression of miR-130a was verified using qRT-PCR. The expression of MDR1 mRNA and P-glycoprotein (P-gp) after cellular transfection was examined using qRT-PCR and western blotting, respectively. Cisplatin sensitivity was detected by the MTT assay.
Drug Response:
resistant
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line (SKOV3,SKOV3/CIS)
Other information
Title:
Altered microRNA expression in cisplatin-resistant ovarian cancer cells and upregulation of miR-130a associated with MDR1/P-glycoprotein-mediated drug resistance.
Journal:
Oncol Rep
Published:
2012
PubMed ID:
22614869