ncRNA basic information
ncRNA ID:
MI0000434
ncRNA Database:
miRBase
ncRNA Name:
Let-7i
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In the present study, we performed miRNA microarray to identify the miRNAs associated with chemotherapy response in ovarian cancer and found that let-7i expression was significantly reduced in chemotherapy-resistant patients . This result was further validated by stem-loop real-time reverse transcription-PCR . Both loss-of-function (by synthetic let-7i inhibitor) and gain-of-function (by retroviral overexpression of let-7i) studies showed that reduced let-7i expression significantly increased the resistance of ovarian and breast cancer cells to the chemotherapy drug, cis-platinum. Finally, using miRNA microarray, we found that decreased let-7i expression was significantly associated with the shorter progression-free survival of patients with late-stage ovarian cancer . This finding was further validated in the same sample set by stem-loop real-time reverse transcription-PCR and in an independent sample set by in situ hybridization .
Drug Response:
resistant
Cancer basic information
Cancer:
epithelial ovarian cancer
Tissue/Cell:
cell line (SKOV3, 2008, OVCAR10, OVCAR3,HeLa,MCF7, MDA-MB-468)
Other information
Title:
MicroRNA microarray identifies Let-7i as a novel biomarker and therapeutic target in human epithelial ovarian cancer.
Journal:
Cancer Res
Published:
2008
PubMed ID:
19074899