ncRNA basic information
ncRNA ID:
MI0000289/MI0000269
ncRNA Database:
miRBase
ncRNA Name:
miR-181a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Bcl-2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00694 (APRD00521)
Drug Name:
Daunorubicin
Drug Method:
MicroRNA microarray and stem-loop reverse transcription-polymerase chain reaction were used to detect the expression of miR-181a. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay was performed to quantify the effect of miR-181a on K562 cells growth and viability. Apoptotic cells were quantitatively detected using Annexin V/FITC and PI apoptosis detection kit. BCL-2 protein expression was measured by western blot. Luciferase reporter vector with the putative BCL-2 3 untranslated region was constructed to explore whether BCL-2 was a direct target gene of miR-181a. BCL-2 siRNA was transfected into the cell to explore the relationship between BCL-2 and DNR resistance.
Drug Response:
sensitive
Cancer basic information
Cancer:
leukemia
Tissue/Cell:
cell line (K562,K562/A02)
Other information
Title:
miR-181a sensitizes a multidrug-resistant leukemia cell line K562/A02 to daunorubicin by targeting BCL-2.
Journal:
Acta Biochim Biophys Sin (Shanghai)
Published:
2012
PubMed ID:
22285729