ncRNA basic information
ncRNA ID:
MI0000342
ncRNA Database:
miRBase
ncRNA Name:
miR-200b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real-Time qRT-PCR
ncRNA Target Gene:
E2F3
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01248 (APRD00932)
Drug Name:
Docetaxel
Drug Method:
The expression levels of miRNA were determined by Real-Time Quantitative Reverse-Transcription Polymerase Chain Reaction and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Analysis of Apoptosis and Cell Cycle. Xenograft Transplantation was uesd to idenntify the relationship between miR-200b and docetaxel resistant.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung adenocarcinoma
Tissue/Cell:
cell line (SPC-A1, A549,SPC-A1/DTX )
Other information
Title:
MicroRNA-200b reverses chemoresistance of docetaxel-resistant human lung adenocarcinoma cells by targeting E2F3.
Journal:
Cancer
Published:
2012
PubMed ID:
22139708