ncRNA basic information
ncRNA ID:
MI0000438
ncRNA Database:
miRBase
ncRNA Name:
miR-15b
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
BMI1
ncRNA Pathway:
transforming growth factor-Beta,Sonic hedgehog-Gli-Snail pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
MicroRNA microarray analyses were performed in CAL27-res cells and CAL27 cells. The expression levels of miRNA were determined by Quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Tumor xenografts was used to identify between miR-200b or miR-15b expression and drug resistant .
Drug Response:
resistant
Cancer basic information
Cancer:
tongue cancer
Tissue/Cell:
cell line (CAL27, SCC25,CAL27-res, SCC25-res)
Other information
Title:
MiR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1.
Journal:
Oncogene
Published:
2012
PubMed ID:
21725369