ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
NA
ncRNA Target Gene:
AP-2alpha
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Using computational programs, we predicted that the 3 untranslated region (UTR) of AP-2alpha gene contains a potential miRNA response element (MRE) for the miR-200b/200c/429 family, and the single nucleotide polymorphism (SNP) site rs1045385 (A or C allele) resided within the predicted MRE. Luciferase assays and Western blot analysis demonstrated that the miR-200b/200c/429 family recognized the MRE in the 3 UTR of AP-2alpha gene and negatively regulated the expression of endogenous AP-2alpha proteins. The effects of the two polymorphic variants on cisplatin sensitivity were determined by clonogenic assay.
Drug Response:
resistant
Cancer basic information
Cancer:
endometrial cancer
Tissue/Cell:
cell line (HEK293,HEC-1A)
Other information
Title:
A miR-200b/200c/429-binding site polymorphism in the 3 untranslated region of the AP-2alpha gene is associated with cisplatin resistance.
Journal:
PLoS One
Published:
2011
PubMed ID:
22194984