ncRNA basic information
ncRNA ID:
MI0000446/MI0000470
ncRNA Database:
miRBase
ncRNA Name:
miR-125b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Bak1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The different expression of miR-125b in cisplatin-sensitive ovarian cancer cell line (OV2008) and its resistant variant (C13*) was identified by real-time PCR. An in vitro cytotoxicity assay and apoptosis assay using CCK-8 assay and flow cytometry, were carried out to detect the effect of miR-125b and Bak1 on cisplatin resistance of cells. Real-time PCR, Western blotting and luciferase reporter assay were used to detect whether Bak1 is a target of miR-125b.
Drug Response:
resistant
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line (OV2008,C13*)
Other information
Title:
miR-125b confers resistance of ovarian cancer cells to cisplatin by targeting pro-apoptotic Bcl-2 antagonist killer 1.
Journal:
J Huazhong Univ Sci Technolog Med Sci
Published:
2011
PubMed ID:
21823019