ncRNA basic information
ncRNA ID:
MI0000447/MI0000727
ncRNA Database:
miRBase
ncRNA Name:
miR-128
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Rap1B
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00853 (APRD00557)
Drug Name:
Temozolomide
Drug Method:
The expression levels of miRNA were determined by real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Wound closure assay and transwell migration assay were performed to examine the capacity of cell migration.
Drug Response:
sensitive
Cancer basic information
Cancer:
glioblastoma
Tissue/Cell:
cell line (U251, U87)
Other information
Title:
miR-128 and miR-149 enhance the chemosensitivity of temozolomide by Rap1B-mediated cytoskeletal remodeling in glioblastoma.
Journal:
Oncol Rep
Published:
2014
PubMed ID:
25017996