ncRNA basic information
ncRNA ID:
ENSG00000234741
ncRNA Database:
Ensembl
ncRNA Name:
GAS5
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
E2F4/PARP1
ncRNA Pathway:
MAPK signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The low expression of lncRNA GAS5 in EOC tissues and OC cell lines was determined by microarray analyses and Real-Time qPCR. Flow cytometer assays were used to detect cell cycle and apoptosis of OC cells. CCK8 assay were performed to investigate the DDP sensitivity of OC cells. Western blot was carried out to detect cell growth markers, apoptotic markers, PARP1, E2F4, MAPK pathway protein expression and other protein expression in OC cell lines. The binding of GAS5 and E2F4 were proved by RNA pull-down and RIP assay. The effect of E2F4 on PARP1 were determined by CHIP-qPCR assay and luciferase reporter assay. The effect of lncRNA GAS5 on OC cells was assessed in vitro and in vivo.
Drug Response:
sensitive
Cancer basic information
Cancer:
epithelial ovarian cancer
Tissue/Cell:
cell line (HEY, A2780, A2780/DDP, HO8910, HO8910PM, SKOV3, SKOV3/DDP,IOSE)
Other information
Title:
Long non-coding RNA GAS5 inhibits DDP-resistance and tumor progression of epithelial ovarian cancer via GAS5-E2F4-PARP1-MAPK axis.
Journal:
J Exp Clin Cancer Res
Published:
2019
PubMed ID:
31391118