ncRNA basic information
ncRNA ID:
ENSG00000251562
ncRNA Database:
Ensembl
ncRNA Name:
MALAT1
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
ZEB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00317 (APRD00997, DB07998)
Drug Name:
Gefitinib
Drug Method:
MALAT1 in A549 and HCC 1299 human lung adenocarcinoma cell lines was silenced by shRNA or overexpressed using plasmid, and the cell viability and cell proliferation were evaluated by MTT assay and soft agar colony formation assay. RNA levels were detected by RT-PCR, and the protein expression was measured by western blot. The binding between MALAT1 and miR-200a was validated by luciferase reporter assays using pSi-Chech 2 vectors.
Drug Response:
resistant
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549, HCC1299)
Other information
Title:
LncRNA MALAT1 Promotes Lung Cancer Proliferation and Gefitinib Resistance by Acting as a miR-200a Sponge.
Journal:
Arch Bronconeumol
Published:
2019
PubMed ID:
31133357