ncRNA basic information
ncRNA ID:
MI0000300
ncRNA Database:
miRBase
ncRNA Name:
miR-223
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
Hsp70
ncRNA Pathway:
JNK signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In the present study, miR-223 expression was down-regulated in OS tissues and cell lines; miR-223 overexpression enhanced the cellular effects of cisplatin (CDDP) on OS cell lines. Through binding to the HSPA1A 3UTR, miR-223 could regulate Hsp70 protein levels and downstream JNK/JUN signaling pathway, thus modulating OS cell apoptosis through Hsp70 under CDDP stress. Finally, JUN, a downstream transcription factor of JNK signaling, could bind to the promoter region of miR-223 to promote its transcription. In summary, miR-223, Hsp70 and downstream JNK/JUN formed a feedback loop to modulate the chemoresistance of OS to CDDP.
Drug Response:
sensitive
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (Saos2, U2OS, HOS, MG63,SJSA-1, SK-ES-1,hFOB)
Other information
Title:
miR-223/Hsp70/JNK/JUN/miR-223 feedback loop modulates the chemoresistance of osteosarcoma to cisplatin.
Journal:
Biochem Biophys Res Commun
Published:
2018
PubMed ID:
29432736