ncRNA basic information
ncRNA ID:
ENSG00000223573
ncRNA Database:
Ensembl
ncRNA Name:
TINCR
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Snail-1
ncRNA Pathway:
miR-125b-HER-2/Snail-1 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00072 (BTD00098, BIOD00098)
Drug Name:
Trastuzumab
Drug Method:
Trastuzumab-resistant SKBR-3-TR and BT474-TR cell lines were established by grafting SKBR-3 and BT474 cells into mouse models and subjected to trastuzumab treatment. LncRNA microarray followed by quantitative reverse transcription PCR (qRT-PCR) was carried out to verify the differentially expressed lncRNAs. Western blotting, bioinformatics analysis, immunofluorescence assay and immunoprecipitation assays (ChIP and RIP) were performed to identify the involvement and functional interactions between H3K27 acetylation and terminal differentiation-induced non-coding RNA (TINCR) or between TINCR and its downstream genes including miR-125b, HER-2 and Snail-1. In addition, a series of in vitro and in vivo assays were performed to assess the functions of TINCR.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (SKBR-3, BT474,MCF-10A)
Other information
Title:
Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast Cancer
Journal:
Mol Cancer
Published:
2019
PubMed ID:
30621694