ncRNA basic information
ncRNA ID:
ENSG00000253352
ncRNA Database:
Ensembl
ncRNA Name:
TUG1
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
The expression levels of lncRNA were determined by Fuantitative real-time PCR (qRT-PCR) .Fuciferase activity assay was used to verify the target genes of lncRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.ChIP experiments were performed using Magna RIP RNA-Binding Protein Immunoprecipitation Kit .Animal experiments was used to test the role of miR-34a and TUG1.
Drug Response:
resistant
Cancer basic information
Cancer:
acute myeloid leukemia
Tissue/Cell:
tissue and cell line (HS-5, HL60)
Other information
Title:
TUG1 confers Adriamycin resistance in acute myeloid leukemia by epigenetically suppressing miR-34a expression via EZH2.
Journal:
Biomed Pharmacother
Published:
2019
PubMed ID:
30551433