ncRNA basic information
ncRNA ID: ENSG00000147687
ncRNA Database: Ensembl
ncRNA Name: TATDN1
ncRNA Type: lncRNA
ncRNA Expression: up-regulated
ncRNA Method: qRT-PCR
ncRNA Target Gene: TRIM66
ncRNA Pathway: NA
Evidence (ncRNA-drug): validated
drug basic information
Drug ID: DB00515 (APRD00359)
Drug Name: Cisplatin
Drug Method: The expression of TATDN1, miR-451 and TRIM66 in NSCLC tissues and cell lines were detected by qRT-PCR or western blot. Immunohistochemistry (IHC) assay was performed for the detection of TATDN1 expression profile. 88 patients who underwent cisplatin treatment were followed up to 60-months for the analysis of survival rate. MTT and Flow cytometry analysis were performed for the assessment of cell survival rate, proliferation and apoptosis. Bioinformatics, Dual-Luciferase reporter were employed to analyze the interaction among TATDN1, miR-451 and TRIM66. Xenograft tumor model was constructed to verify the role of TATDN1 in NSCLC treated with cisplatin (DDP) in vivo.
Drug Response: resistant
Cancer basic information
Cancer: lung non-small cell carcinoma
Tissue/Cell: cell line (A549,H157,A549/DDP,H157/DDP)
Other information
Title: LncRNA TATDN1 contributes to the cisplatin resistance of non-small cell lung cancer through TATDN1/miR-451/TRIM66 axis.
Journal: Cancer Biol Ther
Published: 2019
PubMed ID: 30481109