ncRNA basic information
ncRNA ID:
ENSG00000214548
ncRNA Database:
Ensembl
ncRNA Name:
MEG3
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
NA
ncRNA Pathway:
MEG3-P53 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed to determine cell proliferation. Quantitative polymerase chain reaction was used to determine the levels of MEG3 expressions. Western blot and immunofluorescence were used to detect protein levels. Small interfering RNA or pCDNA-MEG3 transfection was used to downregulate or upregulate MEG3 expression. Dichlorof luorescein diacetate was used to detect intracellular reactive oxygen species. Flow cytometry was used to analyze apoptosis.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549)
Other information
Title:
Paclitaxel promotes lung cancer cell apoptosis via MEG3-P53 pathway activation.
Journal:
Biochem Biophys Res Commun
Published:
2018
PubMed ID:
30173893