ncRNA basic information
ncRNA ID:
ENSG00000234741
ncRNA Database:
Ensembl
ncRNA Name:
GAS5
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
PTEN
ncRNA Pathway:
AKT/mTOR pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00675 (APRD00123)
Drug Name:
Tamoxifen
Drug Method:
A microarray of lncRNAs was screened in tamoxifen-resistant MCF-7R cells and the parental, non-resistant MCF-7-cells. The expression levels of lncRNA were determined by qRT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of lncRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of lncRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. Cell migration was detected by transwell assay. Subcutaneous xenograft mouse model was used to test the role of lncRNA GAS5 .
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7,MCF-7R)
Other information
Title:
Downregulation of lncRNA GAS5 confers tamoxifen resistance by activating miR-222 in breast cancer.
Journal:
Cancer Lett
Published:
2018
PubMed ID:
29969658