ncRNA basic information
ncRNA ID:
ENSG00000149582
ncRNA Database:
Ensembl
ncRNA Name:
TMEM25
ncRNA Type:
lncRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
MRP, BCRP, BCRP, and MDR/p-gp
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
The expression of lncRNA RP11-770J1.3 and TMEM25 in human breast cancer MCF-7(paclitaxel sensitive) and MCF-7/PR(paclitaxel resistant) cells were detected by quantitative RT-PCR. The synthetic interfering fragments of lncRNA RP11-770J1.3 and TMEM25 were transfected into MCF-7/PR cells. Sulforhodamine B assay was used to detect the sensitivity of MCF-7/PR cells to paclitaxel after interference of lncRNA RP11-770J1.3 and TMEM25. The expression of multidrug-resistance genes and proteins were detected by qRT-PCR and Western blot, respectively.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7,MCF-7/PR)
Other information
Title:
Effects of lncRNA RP11-770J1.3 and TMEM25 expression on paclitaxel resistance in human breast cancer cells
Journal:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Published:
2017
PubMed ID:
29256224