ncRNA basic information
ncRNA ID:
ENSG00000130600
ncRNA Database:
Ensembl
ncRNA Name:
H19
ncRNA Type:
lncRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
CBL
ncRNA Pathway:
LncRNA-H19/MiR675-5p Pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
NA
Drug Name:
Huaier extract
Drug Method:
Microarray profiling was performed to identify the candidate lncRNAs affected by Huaier extract. Quantitative realtime PCR (qPCR) was used to evaluate the transfection efficiency and the influence of Huaier extract on H19 expression. The effect of Huaier extract on the cell viability was examined by MTT. Moreover, the rates of apoptotic cells were detected using flow-cytometric analysis. Western blot analysis was applied to show the protein levels of CBL.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MDA-MB-231, MDA-MB-468 and MCF7)
Other information
Title:
Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway.
Journal:
Cell Physiol Biochem
Published:
2017
PubMed ID:
29145193