ncRNA basic information
ncRNA ID:
ENSG00000234741
ncRNA Database:
Ensembl
ncRNA Name:
GAS5
ncRNA Type:
lncRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
miR-21/PTEN pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of lncRNA were determined by Real-time PCR and those of protein were by Western blot analysis.Fuciferase activity assay was used to verify the target genes of miRNAs.MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Soft agar assays were performed to determine the in vitro tumorigenicity of the cells.
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
tissue and cell line ( A549, NCI-H1299, H460, SK-MES-1,H157, H358,16HBE)
Other information
Title:
GAS5 knockdown reduces the chemo-sensitivity of non-small cell lung cancer (NSCLC) cell to cisplatin (DDP) through regulating miR-21/PTEN axis.
Journal:
Biomed Pharmacother
Published:
2017
PubMed ID:
28686971