ncRNA basic information
ncRNA ID:
MI0000077
ncRNA Database:
miRBase
ncRNA Name:
miR-21
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR,Northern blot
ncRNA Target Gene:
PTEN
ncRNA Pathway:
AKT pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00072 (BTD00098, BIOD00098)
Drug Name:
Trastuzumab
Drug Method:
miRNA microarray analysis was done in the parental and resistant BT474 linesThe expression levels of miRNA were determined by Real-time PCR and Northern Blot .Those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs.The nude mice was used to identify the relationship between miR-21 and trastuzumab.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (SKBR3, MDA-MB-453, and BT474)
Other information
Title:
Up-regulation of miR-21 mediates resistance to trastuzumab therapy for breast cancer.
Journal:
J Biol Chem
Published:
2011
PubMed ID:
21471222