ncRNA basic information
ncRNA ID:
NA
ncRNA Database:
Ensembl
ncRNA Name:
lncRNA MRUL
ncRNA Type:
lncRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ABCB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of lncRNA were determined by qRT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of lncRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of lncRNAs. lncRNA microarray was used to test the lncrna expression in different cell lines .Fluorescence intensity assay for intracellular ADR. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (SGC7901,SGC7901/VCR,SGC7901/ADR)
Other information
Title:
Long noncoding RNA MRUL promotes ABCB1 expression in multidrug-resistant gastric cancer cell sublines.
Journal:
Mol Cell Biol
Published:
2014
PubMed ID:
24958102