ncRNA basic information
ncRNA ID:
MI0000443/MI0000444/MI0000445
ncRNA Database:
miRBase
ncRNA Name:
miR-124
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
STAT3
ncRNA Pathway:
STAT3/HIF-1 signaling pathways
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
To understand the inner mechanism of drug resistance in breast cancer and look for remedy methods, we referred to bioinformatic analysis and predicted that signal transducer and activator of transcription 3 (STAT3) and miR-124 was overexpressed in MCF7-R cells (MCF7 cells resistant to DOX) compared with MCF cells. Expression levels of RNA and protein were separately determined by qRT-PCR and western blot. Dual luciferase assay was performed to verify the targeting relationship between STAT3 and miR-124. Optical density (OD) values and apoptotic rates of cells were respectively determined via MTT assays and flow cytometric analysis. Cell invasion was detected to verify drug resistance.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF7)
Other information
Title:
MiR-124 Reversed the Doxorubicin Resistance of Breast Cancer Stem Cells Through STAT3/HIF-1 Signaling Pathways.
Journal:
Cell Cycle
Published:
2019
PubMed ID:
31286834