ncRNA basic information
ncRNA ID:
MIMAT0000432
ncRNA Database:
miRBase
ncRNA Name:
miR-141-3p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
CDK8
ncRNA Pathway:
miRNA-141-3p/CDK8 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00072 (BTD00098, BIOD00098)
Drug Name:
Trastuzumab
Drug Method:
Microarray analysis was performed to screen microRNAs that are differentially expressed in wild type and trastuzumab-resistant (TR) breast cancer cell lines. TargetScan helped predict the target gene of miR-141-3p. The regulatory relationship was confirmed through a luciferase reporter assay, quantitative reverse transcriptase polymerase chain reaction, and Western blot analysis. The MTT assay, transwell invasion assay, and wound scratch assay were performed to measure the proliferative, invasive, and migratory ability of breast cancer cells, respectively. Tumor cell xenografts in nude mice were conducted to observe the effect of miR-141-3p on trastuzumab resistance in breast cancer cells in vivo. The enzyme-linked immunosorbent assay was used to detect protein secretion.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
tissue and cell line (MDA-MB-231, SKBR3, HEK-293T )
Other information
Title:
The microRNA-141-3p/ CDK8 pathway regulates the chemosensitivity of breast cancer cells to trastuzumab.
Journal:
J Cell Biochem
Published:
2019
PubMed ID:
31087707