ncRNA basic information
ncRNA ID:
MIMAT0003948
ncRNA Database:
miRBase
ncRNA Name:
miR-770-5p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
HER2
ncRNA Pathway:
HER2 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00072 (BTD00098, BIOD00098)
Drug Name:
Trastuzumab
Drug Method:
This study aimed to find drug-responsive miRNAs, and explore their anticancer activities in HER2+ breast cancer cells and regulatory role in the trastuzumab response. qRT-PCR-array analysis was performed with effective concentrations of tamoxifen and trastuzumab treated BT-474, SK-BR-3 and MCF-7 cells. Motility and invasion analyses were performed with wound healing and xCELLigence impedance-based assays respectively. Viability of cells following mimic transfection and drug treatment was assessed by WST-1 assay. Western blot analysis was used to assess miR-770-5p regulation of proteins and their phosphorylated forms. The clinical relevance of miR-770-5p was examined by TCGA data analysis.
Drug Response:
sensitive
Cancer basic information
Cancer:
her2-receptor positive breast cancer
Tissue/Cell:
cell lines (BT-474, SK-BR-3, MCF-7)
Other information
Title:
Involvement of miR-770-5p in trastuzumab response in HER2 positive breast cancer cells.
Journal:
PLoS One
Published:
2019
PubMed ID:
31009516