ncRNA basic information
ncRNA ID:
MIMAT0003239
ncRNA Database:
miRBase
ncRNA Name:
miR-574-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
ZEB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
We investigated the mechanism via which miR-574-3p regulated cancer cell migration and invasion to determine the relationship between epithelial mesenchymal transition (EMT) and drug resistance. The expression levels of miRNA were determined by Transient transfection and quantitative real-time PCR (qPCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
gastric carcinoma
Tissue/Cell:
cell line (e SGC7901, AGS, BGC823, MGC803, HGC27, HEK293T)
Other information
Title:
MicroRNA-574-3p regulates epithelial mesenchymal transition and cisplatin resistance via targeting ZEB1 in human gastric carcinoma cells.
Journal:
Gene
Published:
2019
PubMed ID:
30917930