ncRNA basic information
ncRNA ID:
MIMAT0004925
ncRNA Database:
miRBase
ncRNA Name:
miR-876-3p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
TMED3
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Cell viability was analyzed via CCK-8 and colony formation assay. Stem cell-like properties were examined via spheroid colony formation assay. mRNA abundance of key genes was analyzed via quantitative polymerase chain reaction. Protein level of TMED3 and stem cell markers was examined by western blot. TargetScan, luciferase, and biotin-miRNA pulldown assay were used to identify miR-876-3ps target.
Drug Response:
resistant
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell lines (MGC-803, AGS, BGC-823, SGC-7901, MKN-45, N87, HGC-27,GES-1)
Other information
Title:
MiR-876-3p regulates cisplatin resistance and stem cell-like properties of gastric cancer cells by targeting TMED3.
Journal:
J Gastroenterol Hepatol
Published:
2019
PubMed ID:
30843262