ncRNA basic information ncRNA ID: MIMAT0005871 ncRNA Database: miRBase ncRNA Name: miR-1207-5p ncRNA Type: miRNA ncRNA Expression: up-regulated ncRNA Method: RT-qPCR ncRNA Target Gene: LZTS1 ncRNA Pathway: NA Evidence (ncRNA-drug): validated

drug basic information Drug ID: DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281) Drug Name: Taxol Drug Method: The present study first evaluated the expression levels of microRNA (miR)-1207-5p in human normal breast epithelial MCF-10A cells and TNBC cell lines (MDA-MB-231, MDA-MB-436 and MDA-MB-453). The results revealed that the highest miR-1207-5p level was in MDA-MB-231, which suggested an oncogenic role of miR-1207-5p in TNBC. Therefore, MDA-MB-231 served as the present studys research model in subsequent experiments. The mRNAs that functioned as tumor suppressor factors for miR-1207-5p were then determined. Leucine zipper tumor suppressor gene 1 (LZTS1), which was predicted by TargetScan 6.2 and was supported by the results of a dual luciferase assay, was identified as a target of miR-1207-5p. AntagomiR-1207-5p increased LZTS1 mRNA and protein expressions, enhanced cell growth arrest and cell apoptosis induced by Taxol in MDA-MB-231 cells. Additionally, it was observed that, when compared with Taxol treatment, the combination of Taxol and antagomiR-1207-5p induced a sharp decrease in B-cell lymphoma 2 (Bcl-2) and phosphorylated-protein kinase B expression accompanied by an increase in the Bcl-2-associated X protein expression. Finally, miR-1207-5p expression was significantly increased, while LZTS1 expression was significantly decreased, in TNBC tissues when compared with normal adjacent tissues, and there was a negative correlation between miR-1207-5p and LZTS1 expression. In addition, there was a notable elevation in the expression of miR-1207-5p and a reduction in the expression of LZTS1 in the Taxol non-responsive TNBC tissues when compared with the Taxol-responsive TNBC tissues. Drug Response: resistant

Cancer basic information Cancer: triple-receptor negative breast cancer Tissue/Cell: cell line (MCF-10A,MDA-MB-231, MDA-MB-436, MDA-MB-453 )

Other information Title: miR-1207-5p regulates the sensitivity of triple-negative breast cancer cells to Taxol treatment via the suppression of LZTS1 expression. Journal: Oncol Lett Published: 2019 PubMed ID: 30655858