ncRNA basic information
ncRNA ID:
MI0000238/MI0000279
ncRNA Database:
miRBase
ncRNA Name:
miR-196a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
CDKN1B and ING5
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Focusing on CAFs as stromal drivers of chemoresistance, we aimed to investigate the mechanism underlying CAF-mediated cisplatin resistance in HNC.The expression levels of miRNA were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
head and neck cancer
Tissue/Cell:
cell line (SCC-4, SCC-9, SCC-25, CAL 27, 293T, HN4, HN6, HN30)
Other information
Title:
Exosomal miR-196a derived from cancer-associated fibroblasts confers cisplatin resistance in head and neck cancer through targeting CDKN1B and ING5.
Journal:
Genome Biol
Published:
2019
PubMed ID:
30642385