ncRNA basic information
ncRNA ID:
MI0000446/MI0000470
ncRNA Database:
miRBase
ncRNA Name:
miR125b
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
PR-qPCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miR125b in doxorubicine drug-sensitive and doxorubicine drug-resistant leukemia cell lines.HL-60, K562 and HL-60/Dox, the K562/Dox were detected by using RT-qPCR; the up-regulation or inhibition of miR-1256 expression in HL-60/Dox were performed by electroporation transfection, then the viability of cells treated with doxorubicine of different concentration was detected by CCK-8 method, the proliferation inhibition curve of cells was drawed, and the IC50 was calculated.
Drug Response:
sensitive
Cancer basic information
Cancer:
leukemia
Tissue/Cell:
cell line (HL-60, K562 and HL-60/Dox)
Other information
Title:
Down-Regulation of MiR-125b Reverses Drug Resistance of Doxorubicin-Resistant Leukemia Cells
Journal:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Published:
2018
PubMed ID:
30501692