ncRNA basic information
ncRNA ID:
MI0000298
ncRNA Database:
miRBase
ncRNA Name:
miR-221
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
PCR
ncRNA Target Gene:
APAF-1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00317 (APRD00997, DB07998)
Drug Name:
Gefitinib
Drug Method:
PC-9 cells were transfected with LV-NC and LV-miR-221 to establish cell stabilizing strains (PC-9/NC and PC-9/miR-221), then they were used to detect the relative expression of miR-221 and apoptotic protease activating factor-1 (APAF-1) mRNA by real-time fluorescence quantitative PCR (qRT-PCR). The effects of gefitinib (0-4 umol/L) on the growth and proliferation of cell stabilizing strains were detected by CCK-8 Assay. After gefitinib treatment, cell apoptosis was detected by Flow Cytometry Assays. The expression of epidermal growth factor receptor (EGFR), phosphorylated epidermal growth factor receptor (p-EGFR), APAF-1 and cleaved cysteinyl aspartate specific proteinase-3 (Cleaved-caspase-3) were detected by Western blot. Dual-Luciferase Reporter Assay was used to evaluate the relationship between APAF-1 and miR-221
Drug Response:
resistant
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (PC-9/NC,PC-9/miR-221)
Other information
Title:
Mechanism of miR-221 contributes to gefitinib resistance in PC-9 cells
Journal:
Zhonghua Yi Xue Za Zhi
Published:
2018
PubMed ID:
30440142